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| Lossouarn, Julien; Dupont, Samuel; Gorlas, Aurore; Mercier, Coraline; Bienvenu, Nadege; Marguet, Evelyne; Forterre, Patrick; Geslin, Claire. |
| Mobile genetic elements (MGEs) such as viruses, plasmids, vesicles, gene transfer agents (GTAs), transposons and transpovirions, which collectively represent the mobilome, interact with cellular organisms from all three domains of life, including those thriving in the most extreme environments. While efforts have been made to better understand deep-sea vent microbial ecology, our knowledge of the mobilome associated with prokaryotes inhabiting deep-sea hydrothermal vents remains limited. Here we focus on the abyssal mobilome by reviewing accumulating data on viruses, plasmids and vesicles associated with thermophilic and hyperthermophilic Bacteria and Archaea present in deep-sea hydrothermal vents. |
| Tipo: Text |
Palavras-chave: Deep-sea hydrothermal vent; Bacteria; Archaea; (Hyper-)thermophiles; Mobilome. |
| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00260/37145/36731.pdf |
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| Geslin, Claire; Gaillard, Melusine; Flament, Didier; Rouault, Karen; Le Romancer, Marc; Prieur, Daniel; Erauso, Gael. |
| Only one virus-like particle (VLP) has been reported from hyperthermophilic Euryarchaeotes. This VLP, named PAV1 is shaped like a lemon and was isolated from a strain of "Pyrococcus abyssi," a deep-sea isolate. Its genome consists of a double-stranded circular DNA of 18 kb which is also present at a high copy number (60 per chromosome) free within the host cytoplasm but is not integrated into the host chromosome. Here, we report the results of complete analysis of the PAV1 genome. All the 25 predicted genes, except 3, are located on one DNA strand. A transcription map has been made by using a reverse transcription-PCR assay. All the identified open reading frames (ORFs) are transcribed. The most significant similarities relate to four ORFs. ORF 180a shows... |
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| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-3536.pdf |
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| Leulliot, N.; Quevillon-cheruel, S.; Graille, M.; Geslin, Claire; Flament, Didier; Le Romancer, Marc; Van Tilbeurgh, H.. |
| Pyrococcus abyssi virus 1 (PAV1) was the first virus particle infecting a hyperthermophilic Euryarchaeota (Pyrococcus abyssi strain GE23) that has been isolated and characterized. It is lemon shaped and is decorated with a short fibered tail. PAV1 morphologically resembles the fusiform members of the family Fuselloviridae or the genus Salterprovirus. The 18 kb dsDNA genome of PAV1 contains 25 predicted genes, most of them of unknown function. To help assigning functions to these proteins, we have initiated structural studies of the PAV1 proteome. We determined the crystal structure of a putative protein of 137 residues (PAV1-137) at a resolution of 2.2 angstrom. The protein forms dimers both in solution and in the crystal. The fold of PAV1-137 is a... |
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| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00134/24562/22595.pdf |
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| Mercier, Coraline; Lossouarn, Julien; Haverkamp, Thomas; Bienvenu, Nadège; Godfroy, Anne; Cueff-gauchard, Valerie; Geslin, Claire; Nesbo, Camilla. |
| Here, we present the draft genome sequences of two thermophilic Marinitoga strain members of the Thermotogales order, Marinitoga camini DV1155 and Marinitoga camini DV1197. These strains were isolated from deep-sea hydrothermal vents of the Mid-Atlantic Ridge. |
| Tipo: Text |
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| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00357/46833/46708.pdf |
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| Byrne, Nathalie; Lesongeur, Francoise; Bienvenu, Nadege; Geslin, Claire; Alain, Karine; Prieur, Daniel; Godfroy, Anne. |
| Both cultivation and molecular techniques were used to investigate the microbial diversity and dynamic of a deep-sea vent chimney. The enrichment cultures performed in a gas-lift bioreactor were inoculated with a black smoker chimney sample collected on TAG site on the mid-Atlantic ridge. To mimic as close as possible environmental conditions, the cultures were performed in oligotrophic medium with nitrogen, hydrogen and carbon dioxide (N-2/H-2/CO2) gas sweeping. Also, the temperature was first settled at a temperature of 85A degrees C and colloidal sulphur was added. Then, the temperature was lowered to 60A degrees C and sulphur was omitted. Archaeal and bacterial diversity was studied in both culture and natural samples. Through 16S rRNA gene sequences... |
| Tipo: Text |
Palavras-chave: 16S rRNA genes; Oligotrophic condition; Environmental condition; Bioreactor; Continuous enrichment culture; Microbial diversity; Deep sea hydrothermal vent. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6920.pdf |
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| Groisillier, Agnes; Herve, Christiane; Jeudy, Alexandra; Rebuffet, Etienne; Pluchon, Pierre Francois; Chevolot, Yann; Flament, Didier; Geslin, Claire; Morgado, Isabel M.; Power, Deborah; Branno, Margherita; Moreau, Herve; Michel, Gurvan; Boyen, Catherine; Czjzek, Mirjam. |
| Background: The production of stable and soluble proteins is one of the most important steps prior to structural and functional studies of biological importance. We investigated the parallel production in a medium throughput strategy of genes coding for proteins from various marine organisms, using protocols that involved recombinatorial cloning, protein expression screening and batch purification. This strategy was applied in order to respond to the need for post-genomic validation of the recent success of a large number of marine genomic projects. Indeed, the upcoming challenge is to go beyond the bioinformatic data, since the bias introduced through the genomes of the so called model organisms leads to numerous proteins of unknown function in the still... |
| Tipo: Text |
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| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00011/12219/9003.pdf |
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| Lossouarn, Julien; Nesbo, Camilla L.; Mercier, Coraline; Zhaxybayeva, Olga; Johnson, Milo S.; Charchuck, Rhianna; Farasin, Julien; Bienvenu, Nadege; Baudoux, Anne-claire; Michoud, Gregoire; Jebbar, Mohamed; Geslin, Claire. |
| Prokaryotic viruses play a major role in the microbial ecology and evolution. However, the virosphere associated with deep-sea hydrothermal ecosystems remains largely unexplored. Numerous instances of lateral gene transfer have contributed to the complex and incongruent evolutionary history of Thermotogales, an order well represented in deep-sea hydrothermal vents. The presence of clustered regularly interspaced short palindromic repeats (CRISPR) loci has been reported in all Thermotogales genomes, suggesting that these bacteria have been exposed to viral infections that could have mediated gene exchange. In this study, we isolated and characterized the first virus infecting bacteria from the order Thermotogales, Marinitoga piezophila virus 1 (MPV1). The... |
| Tipo: Text |
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| Ano: 2015 |
URL: http://archimer.ifremer.fr/doc/00254/36483/36732.pdf |
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| Haverkamp, Thomas H. A.; Geslin, Claire; Lossouarn, Julien; Podosokorskaya, Olga A.; Kublanov, Ilya; Nesbo, Camilla L.. |
| Thermosipho species inhabit thermal environments such as marine hydrothermal vents, petroleum reservoirs, and terrestrial hot springs. A 16S rRNA phylogeny of available Thermosipho spp. sequences suggested habitat specialists adapted to living in hydrothermal vents only, and habitat generalists inhabiting oil reservoirs, hydrothermal vents, and hotsprings. Comparative genomics of 15 Thermosipho genomes separated them into three distinct species with different habitat distributions: The widely distributed T. africanus and the more specialized, T. melanesiensis and T. affectus. Moreover, the species can be differentiated on the basis of genome size (GS), genome content, and immune system composition. For instance, the T. africanus genomes are largest and... |
| Tipo: Text |
Palavras-chave: Extremophiles; Hydrothermal vents; Mobile DNA; Speciation; Thermotogae; Vitamine B-12. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00478/58934/61503.pdf |
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